The antioxidant activity and hypolipidemic activity of the total flavonoids from the fruit of Rosa laevigata Michx

In the present work, the antioxidant activity in vitro and hypolipidemic activity of the total flavonoids (TFs) from the Rosa laevigata Michx fruit was evaluated, and the antioxidant effect in vivo was also discussed. The TFs exhibited a high scavenging effect on 2, 2-diphenyl-1picrylhydrazyl (·DPPH) with IC50 values 0.01 mg/mL, and a stong reduce power in the test. Hyperlipemic mice were intragastric administrated with TFs (25, 50 mg/kg/day) for 4 weeks, and fenofibrate was used as the positive reference substance. After the experiment, the levels of TC (total cholesterol), TG (triglyceride), LDLC (low density lipoprotein-cholesterol) of the mice administrated with high-dose of TFs were markedly declined by 45.02%, 33.86% and 73.68%, respectively, while HDL-C (high density lipoprotein-cholesterol) was significantly increased compared with model group. To investigate the hepatoprotective effect, histopathological assay, ALT (alanine aminotransferase), AST (aspartate aminotransefrase) and ALP (alkaline phosphatase) were also studied, and the results showed that TFs exhibited a favorable effect on liver protection, of which the levels of ALT, AST and ALP were elevated by 55.85%, 29.15% and 25.68%, respectively. Furthermore, the TFs could significantly decrease the MDA (malondialdehyde) level and improve the levels of CAT (Catalas), SOD (superoxide dismutase), GSH (reduced glutathione), and GPX (glutathione peroxidase) compared with hyperlipemia mice. Our results suggested that TFs has a high antioxidant activity and hypolipidemic activity, which can be used as a potential medicine for cardiovascular diseases.